Duke Functional Genomics
Targeted CRISPR services, custom cell line engineering and sgRNA/Cas9 vector construction.
What they do
- Services
- Targeted CRISPR services
- Custom cell line engineering
- SgRNA/Cas9 vector construction
- High-throughput screening
- Screen design
- Assay development
- Pre-screen validation
- Data analysis
- Custom pooled library assembly
- Library formatting services
- Custom siRNA sets
- Custom lentiviral shRNA gene sets
- Lentivirus preparation
- Individual shRNA clones
- Training and equipment use
- CRISPR
- RNAi
- ORF overexpression
- RNP nucleofection
- Lentiviral transduction
- Transient transfection
- siRNA screening
- shRNA screening
- Compound screening
- Immunofluorescence assays
- ELISAs
- Genome-wide screens
- Plate-based assays
- Pooled screening
- Phenotypic assays
- Custom CRISPR libraries
- Human ORF screening
- Human genome screening
- Chemical library reformatting
- Automated plate filling
- Automated plate washing
- Walk-away functionality