Duke Molecular Genomics Core (MGC)
Tissue block DAPI QC, multimodal Cell Segmentation staining and xenium Analyzer Imaging and output.
What they do
- Services
- Tissue block DAPI QC
- Multimodal Cell Segmentation staining
- Xenium Analyzer Imaging and output
- Post-Xenium H&E staining
- Tissue Optimization Assay
- H&E staining
- Next Gen Sequencing
- Basic data analysis
- Tissue sectioning
- Tissue block RNA extraction/QC
- Tissue block H&E QC
- Slide and library preparation
- Advanced data analysis
- RNA and DNA assessment
- Free cell assessments
- Focused SNP genotyping
- Advanced single-cell analysis
- Advanced spatial analysis
- Chromium scRNAseq/snRNAseq
- Visium transcriptomic spatial gene expression
- Xenium in situ sequencing
- 3’ Single Cell RNAseq
- 3’ Single Nuclei RNAseq
- Flex/Fixed RNA Profiling
- 5’ Single Cell Immune Profiling
- ScATAC-seq
- Multiome ATAC + Gene Expression
- TaqMan SNP Genotyping Assays
- qPCR amplification
- Qubit quantitation assays
- Automated electrophoresis
- Multiomics facility
- Cellanome end-to-end automation
- Live cell phenotyping
- Single-cell dynamics
- Cell-cell interactions
- Cell perturbations
- Cell states and transitions
- Xenium spatial resolution
- Up to 5000 genes per run
- Visium total mRNA analysis
- Custom probe spike-in
- SNP allele detection
- RNA/DNA quantitation
- Nucleic acid quality control